An Iterative Bézier Method for Fitting Beta-sheet Component of a Cryo-EM Density Map

• Autorzy: Michael Poteat , Jing He
• Języki publikacji: EN

Abstrakty

EN

Cryo-electron microscopy (Cryo-EM) is a powerful technique to produce 3-dimensional density maps for large molecular complexes. Although many atomic structures have been solved from cryo-EM density maps, it is challenging to derive atomic structures when the resolution of density maps is not sufficiently high. Geometrical shape representation of secondary structural components in a medium-resolution density map enhances modeling of atomic structures.We compare two methods in producing surface representation of the β-sheet component of a density map. Given a 3-dimensional volume of β-sheet that is segmented from a density map, the performance of a polynomial fitting was compared with that of an iterative Bézier fitting. The results suggest that the iterative Bézier fitting is more suitable for β-sheets, since it provides more accurate representation of the corners that are naturally twisted in a β-sheet.

• Wydawca: De Gruyter Open
• Czasopismo: Molecular Based Mathematical Biology
• Rocznik: 2017
• Tom: 5
• Numer: 1
• Strony: 31-39

Daty

wydano

2017-01-01

otrzymano

2016-10-06

zaakceptowano

2017-04-03

online

2017-04-27

Twórcy

autor

Michael Poteat

• Department of Computer Science, Old Dominion University, Norfolk, VA, 23529,

autor

Jing He

• Department of Computer Science, Old Dominion University, Norfolk, VA, 23529,

Identyfikatory

DOI

10.1515/mlbmb-2017-0003