Tropospheric ozone affects plant growth and the yield of main pasture species all around the world. Experiments are usually performed in fully controlled conditions; the number of investigations in ambient air conditions is still limited. Moreover, most investigations of the effect of ozone on white clover biomass production consider one series after the other, including a period without leaves. Hence, based on the recommendations, additional series are proposed and studied here. The responses of sensitive and resistant white clover clones are presented and compared using multivariate analysis of variance and profile analysis. The canonical variate analysis used here makes it possible to present the profile comparison of dry matter content of white clover graphically in Euclidean space. The investigations revealed a difference in response between clones and the necessity of using the additional series.